Boehringer Ingelheim BioXcellence™ has developed and implemented a spectrum of processes employing yeast hosts. With our expertise we are able to generate suitable fermentation strains and downstream processes in terms of economy, product quality and efficiency.
Furthermore, Boehringer Ingelheim BioXcellence™ has access to the proprietary Pichia pastoris expression system via collaboration with VTU Technology www.vtu-technology.com.
The technology platform is characterized by an unparalleled speed of development, high yields and high-quality results thanks to the application of advanced and exclusive technologies.
The proteins are secreted into the fermentation supernatant. A strong and inducible AOX promoter and variants of it maximize expression of more than 10 g/l. This technology is best suited for the expression of e.g. Nanobodies® and HSA fusion proteins.
The key features of the Pichia pastoris expression system are as follows: